Virological profile of pregnant HIV positive women with high levels of CD4 count in low income settings: Can viral load help as eligibility criteria for maternal triple ARV prophylaxis (WHO 2010 option B)?

Virological profile of pregnant HIV positive women with high levels of CD4 count in low income settings: Can viral load help as eligibility criteria for maternal triple ARV prophylaxis (WHO 2010 option B)?

 

Anne Esther Njom Nlend^1,2,&, Chantal Same Ekobo^1,2, Suzie Tetang Ndiang Moyo¹, Gisele Chewa Nguetcheng¹, Peter Ngang¹, Sidonie Lyeb^1,2, Luc Meka¹, Martin Baane¹

 

¹National Social Insurance Fund Hospital, Yaoundé, Cameroon, ²Association Camerounaise d’Aide aux Personnes et Familles Affectées par le SIDA (ACAPFAS), Yaoundé, Cameroon

 

 

^&Corresponding author
Anne Esther Njom Nlend, National Social Insurance Fund Hospital –Association Camerounaise d’Aide aux Personnes et Familles Affectées par le SIDA (ACAPFAS)-PO BOX 5777 Yaoundé, Cameroon

 

 

Sub-Saharan Africa continues to record the highest burden of the HIV-1 epidemic, contributing more than 90% in the new cases of perinatally acquired HIV pediatric infections [1]. Cameroon unfortunately is listed among the 20 countries most affected by the HIV pediatric pandemic given the number of HIV pregnant women expected yearly [2]. Mother-to-child transmission of HIV is known to be correlated to maternal viral load, and maternal HIV-1 RNA load has been shown to be the strongest predictor of vertical transmission. In fact, the rate of MTCT has been estimated at 0.3% when maternal viral load is < 1000 copies/ml), at 3% between 1000-10000 copies /ml and at 7% when it is > 10000 copies/ml [3-5]. Despite such evidence, maternal viral load has never been systematically measured in pregnant women in Cameroon, neither to determine the burden of primary acute infection in pregnancy nor to determine the best antiretroviral protocol. The aim of this study was to describe the virological profile of pregnant women newly diagnosed as HIV infected and unaware of treatment.

 

 

Study Population and Procedures

 

The study population was an observational cohort of pregnant HIV positive women. Pregnant women with < 36 weeks of amenorrhea were enrolled in 25 antenatal clinics in the Djoungolo Health District. After opt-out counseling, serial HIV-1/2 algorithm antibody tests were done using Determine HIV-1/2 assay(Abbott Diagnostics, Hoofddorp, the Netherland) and SD bioline HIV 1/3 3.0 (Standard diagnostics Inc, Kyonggi-do, South Korea) rapid kits on mothers´ serum samples. For all HIV positive women, CD4 cell counts were measured in the referral laboratory. The method used a flow cytometer for measuring the percentage of CD4 T cells; an automated blood cell counter helped to measure the total number of lymphocytes. Results were produced in absolute numbers and in percentages. CD4 cell count screening was followed by non exclusive measurement of viral load when CD4 cell counts were > 350/mm³. Maternal baseline serum samples were quantified for HIV-1 RNA load using real Time reverse transcriptase PCR (Generic HIV viral load, Biocentrics). All the samples were analyzed with the Laboratoire Centre Pasteur in Yaoundé acting as service provider at a unit cost of 30US$. ZDV was started for all the others non eligible for triple antiretroviral treatment at 14 weeks of pregnancy, while waiting for the result of the viral load.

 

Data collection and Statistical Analysis

 

The following qualitative variables were considered: maternal age, WHO clinical stages. Quantitative variables included: CD4 cell count and HIV Viral load. Viral load values were log10 transformed and stored in an Excel sheet form. A threshold of ≤ 1.8 log₁₀ was considered as undetectable viral load. For this descriptive analysis we considered (frequency, percentage, medians with their quartile). A confidence interval of 95% was considered as the margin for percentage accuracy.

 

Ethics and administrative aspects

 

All interventions within this project were implemented under the supervision of the District Office of the Ministry of Public Health. Data collection and monitoring of the intervention was part of the routine activities of the Health District. Free measurement of HIV1 RNA viral load was offered to all the eligible mothers at the referral center where all activities were coordinated by the Center for HAART treatment and the local board in charge of fighting against HIV and AIDS.

 

 

Demographic characteristics of the pregnant women

 

Mothers´ mean age (SD) was 27.0 years IQR 22.5-30 and 15/172(8.7%) were adolescents. All the mothers were classified in WHO clinical stage 1 or 2.

 

Immunological profile

 

Median CD4 cell count was 534 cell/mm³, IQR (442-646) and 68/170 had CD4 cell counts of between 350 and 500/mm³.

 

Virological profile

 

Maternal viral load and transmission

 

Table 1 resumes the distribution of the viral load of our positive pregnant women. 126/145 (87%) had detectable serum HIV-1 RNA loads ranging from 1.9 to 6.5 log₁₀. The median log₁₀ viral load was 4.4(IQR 3.5-4.9). 13%, 95% CI (7.5-18) had an undetectable viral load. A viral load of > 3log₁₀ was recorded for 107/145(74 %); 89/145(61%) had a viral load of ≥ 4log₁₀ and for 29/145 (20%, 95% CI 13.5-26.5) a very high level of viral load as usually observed during primary acute infection, was noted ( ≥ 5log₁₀).

 

 

To our knowledge, this is the first study in Cameroon where viral load is systematically determined in pregnant women in order to assess the eligibility to more effective antiretroviral regimen especially at high levels of CD4 cell count. Our key finding is that the level of HIV viral load is ≥ 4log 10/ml in more than 60% of HIV pregnant women, ignorant of HAART, with CD4 cell count > 350/mm³, making them eligible for maternal triple ARV prophylaxis as required elsewhere. Taking into account that an average of 4 in 10 HIV positive pregnant women are yet to be eligible for HAART because of CD4 cell count of < 350/mm³, it may be deduced that among the remaining 6 in 10, at least 4 may require HAART for prophylaxis according to their HIV viral load. Altogether 8 in 10 women at least may be eligible for HAART during pregnancy based upon biological criteria. Such results vindicate the opinion of those who claim that universal access to maternal triple ARV prophylaxis during pregnancy regardless of CD4 cell count is a must and are consistent with the latest WHO recommendations for women not yet in need of treatment for themselves, namely option B [6-8].

 

In Sub-Saharan Africa, some countries have opted for triple antiretroviral therapy for all pregnant women according to the level of care [9]. In the dream cohort, generalizing access to such an approach has led to virtual elimination of MTCT [10]. Option B is recommended without HIV RNA viral load as a prerequisite, but unfortunately many countries affected by generalized epidemic have not been able to opt for such an approach due to cost implications. In the context of option A, access to HIV viral load even at cost recovery rate could help for better management of the target population, notably in HIV care and treatment referral centers.

 

 

We concluded that antenatal serum HIV-1 RNA viral load, stands above 4log₁₀ in more than 60% newly diagnosed pregnant women in Cameroon with high levels of CD4 cell count. Such results suggest the promotion of the routine measurement of viral load during pregnancy in A option country or the switch to B option for all, in order to ensure larger access to maternal antiretroviral triple prophylaxis. This approach is likely to be more effective in lowering maternal viral load and reducing mother-to-child transmission of HIV.

 

 

This study was sponsored by the French Foundation GlaxoSmithKline.

 

 

AENN collected data, carried out the analysis and drafted the manuscript, CSE, STN collected data, GCN, PN, LS, LM, and MB participated in designing the study. All authors read the final version of the manuscript.

 

 

We gratefully acknowledge the women who participated in this study and the support staff of the antiretroviral treatment center. Special mention goes to the French Foundation GlaxoSmithKline for funding this project.

 

 

Table 1: Distribution of HIV-1 RNA load in HIV positive pregnant women with CD4 cell count >350/mm3

 

 

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