Ameloblastic carcinoma: a clinicopathologic analysis of cases seen in a Nigerian Teaching Hospital and review of literature
Olujide Oladele Soyele, Kehinde Emmanuel Adebiyi, Olufunlola Motunrayo Adesina, Adeola Mofoluwake Ladeji, Adetayo Aborisade, Abiodun Olatunji, Henry Ademola Adeola
The Pan African Medical Journal. 2018;31:208. doi:10.11604/pamj.2018.31.208.14660

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Ameloblastic carcinoma: a clinicopathologic analysis of cases seen in a Nigerian Teaching Hospital and review of literature

Cite this: The Pan African Medical Journal. 2018;31:208. doi:10.11604/pamj.2018.31.208.14660

Received: 21/12/2017 - Accepted: 30/10/2018 - Published: 27/11/2018

Key words: Ameloblastoma, ameloblastic carcinoma, odontogenic tumors, cytological atypia, sub-saharan Africa

© Olujide Oladele Soyele et al. The Pan African Medical Journal - ISSN 1937-8688. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Available online at: http://www.panafrican-med-journal.com/content/article/31/208/full

Corresponding author: Henry Ademola Adeola, Division of Dermatology, Department of Medicine, Faculty of Health Sciences and Groote Schuur Hospital, University of Cape Town, Observatory 7925, Cape Town, South Africa (henry.adeola@uct.ac.za)


Ameloblastic carcinoma: a clinicopathologic analysis of cases seen in a Nigerian Teaching Hospital and review of literature

Olujide Oladele Soyele1, Kehinde Emmanuel Adebiyi2, Olufunlola Motunrayo Adesina1, Adeola Mofoluwake Ladeji2, Adetayo Aborisade3, Abiodun Olatunji3, Henry Ademola Adeola4,5,&

 

1Department of Oral Maxillo-facial Surgery and Oral Pathology, Obafemi Awolowo University, Ile-Ife, Nigeria, 2Department of Oral Pathology and Oral Medicine, Faculty of Dentistry, Lagos State University College of Medicine, Ikeja, Lagos, Nigeria, 3Department of Oral and Maxillofacial Surgery and Oral Pathology, Obafemi Awolowo University Teaching Hospital Complex, Ile-Ife, Nigeria, 4Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, University of the Western Cape and Tygerberg Hospital, Cape Town, South Africa, 5Division of Dermatology, Department of Medicine, Faculty of Health Sciences and Groote Schuur Hospital, University of Cape Town, Cape Town, South Africa

 

 

&Corresponding author
Henry Ademola Adeola, Division of Dermatology, Department of Medicine, Faculty of Health Sciences and Groote Schuur Hospital, University of Cape Town, Observatory 7925, Cape Town, South Africa

 

 

Abstract

Introduction: ameloblastic carcinoma is a rare malignant odontogenic neoplasm that exhibits histological features of ameloblastoma in combination with cytological atypia. It may arise de novo or secondarily through malignant de-differentiation of pre-existing ameloblastoma or odontogenic cyst. Secondary ameloblastic carcinomas often results from repeated surgical intervention, which is a mainstay of odontogenic tumor management in resource limited settings. To date, relatively few cases of ameloblastic carcinomas have been reported and many cases have been misdiagnosed as ameloblastoma. This is due to its wide range of clinicopathological feature which range from indolent to aggressive. It may present as an aggressive ulcerated mass or as a simple cystic lesion; hence, it often challenging to delineate from its benign counterpart, ameloblastoma.

 

Methods: this study reviewed the clinicopathological data on 157 cases of odontogenic tumors diagnosed over a 10 years period from the pathology archive of the Oral Pathology Unit of Obafemi Awolowo University Teaching Hospital Complex (OAUTHC), Ile-Ife, Nigeria.

 

Results: of all these cases, we identified that 64.9% were Ameloblastomas, while 8.3% were ameloblastic carcinomas. Primary subtypes of ameloblastic carcinoma constituted 23.08%, while 69.23% of the cases were of the secondary subtype. We also found that the secondary subtype of ameloblastic carcinomas showed a higher mean duration value of 7.7 years. Most lesions were found in posterior mandible and presented with ulceration, perforation and ill-defined borders radiographically.

 

Conclusion: this study is among the few that have documented higher frequency of secondary ameloblastic carcinoma in the scientific literature.

 

 

Introduction    Down

Ameloblastoma is a slowly-growing, locally-invasive epithelial odontogenic tumour and has been regarded as the most common odontogenic tumour in many studies [1-3]. However, other studies have documented it as the second most prevalent odontogenic tumor after the odontomas [4, 5]. It has a reported prevalence of about 1-3% of all jaw tumors and cysts, with varied geographical prevalence ranging from 11-24% of all odontogenic tumors in North America to an estimated global incidence of 0.5 cases per million person-years [6, 7]. Ameloblastoma constituted between 66%-99% of odontogenic tumors in sub-Saharan Africa, with the mandible being more commonly affected (in about 80% of cases) [6, 8]. Ameloblastoma rarely exhibit malignant transformation, as evidenced by the low frequency of occurrence of Ameloblastic carcinoma (AC). More than 100 cases of AC have been reported in the scientific literature, many of which are case reports; hence, there is currently a paucity of information on AC [9-11]. Controversy and lack of consensus still trails AC with regards to its nomenclature and prognosis, due to the relative rarity of this lesion; however, updated classification of Head and Neck tumors by World Health Organization in 2005 seem to have resolved this issue [12]. In this classification, AC was included as one of the odontogenic malignancies. Epithelial malignant odontogenic tumors in this category were: metastasizing (malignant) ameloblastoma (MA), ameloblastic carcinoma (AC), primary intraosseous squamous cell carcinoma (PIOSCC), clear cell odontogenic carcinoma (CCOC) and ghost cell odontogenic carcinoma (GCOC).

 

Earlier on (in 1982), Elzay et al. proposed a classification in which carcinomas occurring within the jaw bones (excluding cases of invasion from the oral mucosa, distant metastasis, and salivary gland) were classified based on the histological evidence of origin thereby describing AC as tumors having the characteristics of ameloblastomas and squamous cell carcinomas [13]. Malignant (metastasizing) ameloblastoma and ameloblastic carcinoma are two distinct malignant variants of ameloblastoma. While AC arises either de novo, from ex-ameloblastoma or from odontogenic cyst [14]; malignant ameloblastoma is entirely an ameloblastoma with probable capacity to metastasis [10, 15]. AC as an odontogenic malignancy, combines the histological features of ameloblastoma with cytological atypia, even in the absence of metastases [13]. It can be further subdivided into the primary and secondary subtypes [8]. The primary subtype, otherwise known as de novo, presents malignant neoplasm ab initio; whereas, the secondary subtype demonstrates a malignant transformation observed in a previously existing ameloblastoma, independent of the presence or absence of metastasis [8, 16]. There is further division of the secondary type of AC into two subtypes namely the intraosseous type arising within a preexisting benign intraosseous ameloblastoma, and the peripheral type, arising within a benign peripheral ameloblastoma [12]. Metastasizing (malignant) ameloblastoma, is known as a tumour that retains its benign histological features regardless of the site of metastasis [9].

 

Ameloblastic carcinoma (AC) is very rare, nevertheless it is the most common malignancy of odontogenic tumours (OTs) and presents a higher incidence compared to that of malignant ameloblastoma by a ratio of 2:1 [7, 17]. The clinical features and biologic behavior of AC are seemingly identical to ameloblastoma in terms of associated bony swelling, tooth mobility and multilocular radiographic presentation, nevertheless they can be distinguishable on the basis of thorough clinical examination and associated features such as attendant pain, ulceration, rapid rate of growth and cortical bone perforation with or without soft tissue invasion, which are often suggestive of malignancy [9, 17]. Meticulous microscopic evidence of cytological atypia with presence of mitosis confirms the diagnosis of AC, despite the observation of classical histopathological features of benign ameloblastoma. AC is characterized by diverse histopathological features which presents a diagnostic dilemma, hence proper evaluation of the patients and extensive analysis of the surgical sections of specimen is advocated. The relative frequency of AC is less than 1% of all malignant neoplasms in the Head and neck region (H&N) [18]; and despite its low occurrence, its diagnostic dilemma necessitates highlighting of its essential clinical behavior and microscopic presentation. This is very important in oral pathology practices in sub-saharan regions of Africa, where human and financial resources are limited. Furthermore, advanced studies in immunohistochemistry and genetics can also facilitate precise diagnosis of AC [10]. Currently, there is dearth of report on AC from Africa, and majority of available studies in the scientific literature are cases reports from Asia and America [9-11, 19]. Hence, the present study aims to highlight the clinical features and histopathologic behavior of AC in a tertiary health centre in Nigeria.

 

 

Methods Up    Down

This study reviewed the clinicopathological data on AC in relation to other odontogenic tumors (OTs) over a 10-year period (2007-2016), from the surgical pathology archive of the Oral Pathology Unit, of the Department of Oral Maxillofacial Surgery and Oral Pathology, Obafemi Awolowo University Teaching Hospital Complex (OAUTHC), Ile-Ife, Nigeria.

 

Clinicopathological details: OTs that was designated as AC in accordance to WHO (2005) classification with adequate information was selected for the study. Information regarding the bio data of the patients (age, gender and site, duration of the lesion, signs and symptoms (specifically swelling, pain, ulceration and cortical bone perforation), radiographic features, type of AC (primary or secondary) and recurrence were obtained from the hospital record and summarized in Table 1.

 

Histopathological reports: histopathology reports were obtained from biopsy record books of the department. Haematoxylin and Eosin (H & E) stained slides of diagnosed ACs were retrieved for re-evaluation and re-confirmation. Cases that presenting doubtful microscopic observations were revisited by obtaining newly prepared H & E slides from formalin-fixed paraffin-embedded (FFPE) blocks and reevaluated for confirmation of diagnosis wherever necessary. Histopathology features obtained are summarized in Table 2. The histopathology parameters were graded by dividing each of the two slides into four quadrants using a slide pen, this is done for each lesion at the centre of the mounted specimen (at X10 Magnification). A score of 25 was assigned per quadrant based on the parameters (hence, making up 100% for each slide). This score was recorded for individual lesions as follows; occasional finding + (25%); frequently seen ++ (25%-50%); dominant +++ (≥ 50%).

 

Quality assurance: two independent certified pathologists carried out all slide examination and the average score was taken as the final score for each lesion as summarized above (Table 2).

 

Statistical analysis: data was analyzed using Stata (MP 13.1 for MacOS StataCorp, Lakeway Dr, College Station, Texas, USA).

 

 

Results Up    Down

A total of 790 cases of Oral and jaw lesions were seen within the 10-year study period. Of these, 157 (19.9%) cases of OTs were found out of which 102 (64.9%) cases were Ameloblastomas. ACs constituted 13 (1.64%) cases of the total biopsy and 8.3% of OTs. Table 1 summarizes the demography of ACs in this study. Three cases were primary subtypes of AC, constituting 23.08%, while 9 (69.23%) cases where of the secondary subtype. It was observed that the secondary subtypes had a higher frequency of occurrence. One case could not be sufficiently accounted for; hence, it was not categorized either as primary or secondary AC subtype.

 

Patient demographics: the mean age of the 13 cases analyzed in our study was 36.8 years (± 15.5), with the age range between 5 and 60 years, and a peak age incidence in the 7th decade of life. Most of the cases (61.5%) were observed within the 4th and 5th decades of life. Mean age duration for males was 42.25 years (± 8.46) and 28.2 years (± 21.07) for females. Analysis showed male preponderance of 8 (61.54%) with a male: female ratio of 1.6:1 (Figure 1). Mean age for cases in the mandible was 37 years, which is approximately equal to that of the maxilla (36.3years), hence showing no significant difference.

 

Duration: the overall mean duration prior to presentation was about 6years (5.99 yrs), ranging from 4 months to 16 years. The secondary type showed a higher mean duration value of 7.7 yrs.

 

Location of lesions: mandible was the most frequent site of affectation, accounting for 10 (76.9%) cases. We observed a mandible: maxilla ratio of 3.3:1. Furthermore, majority of the cases (69.2%) were seen in the posterior regions of either jaw (Figure 2 A).

 

Clinical features: swelling was consistently seen in all cases (100%), and pain with/without ulceration was present in (61.54%) (Figure 2 B and Figure 3, A-D). Bone perforation was seen in 8 (61.54%) cases and two recurrent cases were seen after the initial surgical treatment.

 

Radiographic findings: Plain radiographs generally showed mixed radiolucency/radioopacity, with more of multilocular appearance in some areas (Figure 3 D, F). Well-defined margin was observed in 4 (30.77%) cases, which was lower than cases with ill-defined borders 9 (69.23%).

 

Histology: histologic features mimicking conventional ameloblastoma predominantly follicular growth pattern was seen in about 76.9%, as against 25.6% of plexiform pattern. Presence of peripheral palisading of the basal cell was dominant with 71.8% and reverse polarity of the nuclei were seen in 56.4% of all the cases. There was minimal to low quantity of loose stellate reticulum-like cells (33.3%). Basilar hyperplasia and tumour crowding centrally constituted 66.7% and 61.5%, respectively. Mitotic figures were seen in appreciable areas, constituting 56.4%, while necrosis and clear cells were found in 41.7% and 46.2%, respectively. Keratin pearl formation was seen in 28.2%, while ghost cell was absent in all of our cases. Perivascular invasion was only present in 15.4% of cases. Cytological atypia including pleomorphism, abnormal chromaticity and reverse nuclear/cytoplasmic ratio were frequently observed. Overview of histological finding is provided in Figure 4 and Figure 5.

 

 

Discussion Up    Down

The term ameloblastic carcinoma (AC) was introduced by Shafer et al., (1974) to describe a tumor arising from malignant transformation of epithelial cells of an ameloblastoma [20]. Despite the rarity of AC compared to ameloblastoma, AC is the most common malignant OT. The pathophysiological mechanism of its evolution is still poorly understood, and this has resulted in a lot of controversy about its biology [11, 19]. Sequential carcinogenesis in ameloblastoma has been suggested to involve long term accumulation of multiple genetic defects. This could also be due to a spontaneous mechanism following repeated therapies which may eventually lead to aggressive clinical manifestation [9, 21]. Primary types of AC have been widely reported to be more common than the secondary type [8, 9], however finding from this study contradicts this observation. We found the secondary type being more frequent with a remarkable ratio of 3:1. Furthermore, the overall mean duration at the time of presentation was 5.9 years for primary AC, as compared with the secondary type which was 7.7 years. The range of occurrence of secondary AC in our study was 4 months to 16 years. This alludes to the fact that most of our cases arose from carcinomatous dedifferentiation of a pre-existing intraosseous ameloblastoma (as evident from the initial documented histologic diagnosis of ameloblastoma). To the best of our knowledge, this is one of the few studies of AC from the sub-Saharan African region where secondary AC is more frequent than the primary type. A possible factor attributable to this is patients' attitude. Most patients tend to delay in seeking orthodox medical treatment (as indicated in the mean year of duration) and there are limited resources for timely surgical intervention. Zarbo et al. (2003), submitted that malignant transformation may take up to 10yrs to occur [22], and this is consistent with what we found in our study. Repeated surgical trauma with induction of chronic inflammation was also noted in this study as some of our patients were exposed to multiple biopsies prior to surgery. The high occurrence of AC relative to OTs observed in this study is in contrast with the report from Ladeinde et al. (2005) and Jing et al. (2007) both presenting an incidence of 2.2% and 1.6% from 391 and 1642 OTs, respectively [7, 17]. There is no evidence yet to ascertain this disparity, however it should be noted that this study retrospectively reviewed cases seen within a decade, in comparison to the previous studies where overall cases of OTs were reviewed [7, 17].

 

Demographically, AC presents diverse clinical features most especially in terms of age, gender and site [11, 23]. Findings from the literature showed that it occurs in an average age range of 30-50 years [6], which concurs with our finding. Our study recorded a mean age of 36.8 years (± 15.5) with a range of 5-60 year which was comparable to that observed in other studies [23-25]. It is however lower than mean ages recorded in some studies [9, 11, 26, 27], thus confirming the reported variation in the mean age of the patients diagnosed with AC. Dhir et al. (2003), in their review of 18 cases of ACs observed a higher mean age for maxillary lesions, as compared to the mean ages reported for mandibular lesions [28]. Their findings suggest that maxillary lesions probably occurs in slightly older patients as compared to mandibular ones. Similar reports were documented by Yoon et al. (2009), where a mean age of 56.7 years for maxillary and 49.1 years for mandibular lesions was documented [11]. Although these results were at variance with our findings where maxillary and mandibular cases showed almost equal mean ages (36.3 yrs and 37 years, respectively); this might not be unconnected to the low number of maxillary cases (n=3) in our study. Hence, a larger study could probably substantiate this observation. The gender predilection in our study is predominantly male, although some studies have reported slightly varied findings of which no apparent gender predilection was observed [6, 24]. The general consensus from most studies is higher frequency of occurrence in males [9, 11, 26]. Findings from our study of male to female ratio of 1.6:1 concur with general observation from previous studies. This higher male predilection of AC follows the distribution pattern of its benign counterpart, ameloblastoma [6]. Although mandible is the preferred site for AC as seen in our study, however, a systematic review has shown equal predilection for both jaws [9]. In addition, 69.2% of our cases involved the posterior region of either jaw which is in accordance with other studies [9, 11]. AC presents more aggressive biologic behavior and the most commonly observed characteristics are swelling often with pain, rapid growth and cortical perforation [11, 27]. Trismus, gingival bleeding, paresthesia, epistaxis and dysphonia have also been mentioned [24].

 

All our cases presented with obvious clinical swelling often associated with pain however, it is pertinent to note that painless swelling can be encountered sometimes as observed in about 38.5% of our cases, and this can masked the suspicion of a malignant lesion. Many studies in the scientific literature are in agreement with our finding of some AC presenting a painless swelling [11, 15, 24, 29]. Other features include rapid growth, perforation of the cortex with ulceration of the soft tissue as observed in 61.5% of cases in this study. Multilocular and sometimes unilocular radiolucency has been described as a common radiographic presentation of AC which is consistent with that of ameloblastoma. However, the margin of AC is often ill-defined with occasional presence of focal radio-opacity [15]. Likewise, this is the most frequent observation in this study, as 69.2% presented with decorticated border. This is also consistent with other reports [24, 30, 31], but contradicts the findings by Yoon et al. [11], where 80% presented well defined borders. This therefore portend that AC may demonstrate divergent radiological findings. Advanced imaging technique like CT scan and MRI could provide more precise outcomes; albeit, not many centers in sub-Saharan Africa have access to these instrumentations, routinely. Due to the rarity of AC, precise histopathological diagnostic features have been elusive [20, 32]. However, researchers have highlighted significant microscopic features that could aid in the diagnosis of AC. Akrish et al, in their 20 year review of 37 cases of AC, concluded that histologic evidence of ameloblastoma-like lesion with some areas demonstrating cytological atypia (including cellular and nuclei pleomorphism, hyperchromatism, enlarged nucleoli, abnormal and increased mitosis, and mitotic figures) should alert the pathologist to the possible diagnosis of AC [19, 24, 27]. Furthermore, other histological parameters including central cell spindling, minimal presence or lack of stellate reticulum-like area, squamous metaplasia with keratinization, reversal of nuclei polarity, crowding/hyperplasia of the basal cells, necrosis, neural and/or vascular invasion and appreciable presence of clear cells have also been mentioned as essential clues to the diagnosis of AC [11, 12, 24].

 

As seen in our study, the tumour islands exhibited both plexiform and follicular growth pattern which conforms with observations from previous studies [19, 29, 32, 33]; however, the follicular type was very dominant in our cases which agrees with other studies [19, 29, 33]. Basal cells palisading and reverse polarity of the nuclei were present in all 13 cases. Also present in our AC cases were, nuclear hyperchromatism, spindle-like cells proliferation, crowding of the cells towards the central region, and multilayered basal cell. Furthermore, increased presence of abnormal mitotic cells, cellular and nuclear pleomorphism and hyperchromatism were seen in almost all the AC cases. These findings are consistent with reports in literature [9, 30, 34]. Kamath et al. [35], categorized AC into four types based on the predominant histologic cell viz; ameloblastic cell type, granular cell type, clear cell type and spindle cell type. All except granular cell type were present in our study. Tumor necrosis was found in 48.7% of our cases as seen in the study by Casaroto et al. [29], where they found a total of 25% tumor necrosis and 6.3% ghost cells in 32 cases. Hence, tumor necrosis and presence of ghost cells may be important diagnostic indicators for AC. Although neurovascular invasion were scarce in our study, it does not exclude the diagnosis of AC [24, 27, 35]. The use of immunohistochemistry and genetics has also been known to improve the diagnosis of AC [36, 37]. Yoon et al. 2011 [37] compared the expression patterns of immunohistochemical markers in 10 cases of ameloblastoma and 7 cases of ameloblastic carcinoma, with observation that AC showed a statistically significant higher value in the expression of CK18, parenchymal MMP-2, stromal MMP-9 and Ki-67, in comparison to Ameloblastoma.

 

In another study, Abdelhamed et al. [38] investigated the expression of midkine and Ki67 immunomarkers in ameloblastoma and AC. The study established that midKine and Ki-67 expression were significantly higher in AC than in ameloblastoma. Furthermore, use of Ki67 and AgNOR to differentiate AC from ameloblastoma showed that these markers were highly expressed in AC [29]. In addition, Lei et al. [39] also attempted to differentiate AC from ameloblastoma and atypical ameloblastoma with the use of SOX2 marker (sex determining region-Y-related high mobility group box 2), which is a protein that is involved in ameloblastic epithelial lineage. The study revealed that SOX2 showed a diffuse and strong nuclear staining pattern with 86.4% specificity and 76.9% sensitivity in AC as compared to the benign counterpart. It can therefore be suggested from the immunohistochemistry studies that differential expression of these markers could be used as an ancillary diagnostic tool for AC. Not least, molecular analysis as used in a study has also highlighted a statistically significant presence of p16 mutation (cyclin dependent kinase inhibitor 2A) in all cases of AC samples as compared to observation in only one sample of ameloblastoma [40]. Therefore, it is presumed that p16 alteration may play a role in the malignant progression of AC. Muller et al. investigated nuclear chromosomal ploidy to differentiate between ameloblastomas and AC with reported observation of aneuploid chromosomes in ACs and diploid chromosomes in ameloblastomas [41]. Furthermore, advanced imaging technique using positron emission tomography (PET) and magnetic resonance imaging (MRI) have been used to distinguishing AC from benign Ameloblastoma [42]. In this study, preoperative 18F-α-methyl tyrosine positron emission tomography and magnetic resonance imaging was utilized in the identification of malignant region in AC, although the result was inconclusive.

 

Because of the rarity of large clinical series and long-term follow-up, treatment protocol for AC is debatable. Some clinicians prefer radiotherapy [28], while others advocate for surgical treatment [13, 24]. Ramadas et al., [43] also considered chemotherapy such as use of cisplatin, adriamycin, and cyclophosphamide to be beneficial in cases of unresectable lesion and as post-surgical adjuvant treatment. AC is considered to be radioresistant by some authors and its use is therefore restricted to pre/post-surgical management, cases not suitable for surgical management, as well as in those that exhibit advanced local or metastatic disease [31]. Wide local excision with about 2cm safety margin (with or without adjuvant radiotherapy and or chemotherapy) has been considered as the mainstay of treatment of AC. Although variable successes have been recorded with radiotherapy or surgery either in combination or alone. Role of chemotherapy in the management of AC is still debatable hence it has not been indicated as a primary treatment mode [9]. The benefit of neck dissection is controversial in the absence of evidence of metastasis [30]. Ion beam therapy has also been employed in the management of AC with proven effectiveness and success, most especially in a recurrent lesion or complex surgical case with proximity to vital structures [44]. Although many targeted precision therapies for odontogenic malignancies are still in clinical trials, BRAF V600E inhibitors (e.g. Vemurafenib) and combination checkpoint inhibitors [45, 46], have also been experimentally used for targeted treatment of ameloblastoma and AC.

 

 

Conclusion Up    Down

AC is a rare malignant lesion that demonstrates a spectrum of demography, clinicopathologic features and biologic behavior that ranges from indolent/benign to aggressive/malignant, as observed in this study. Due to the rare nature of AC, it often presents diagnostic dilemma to many oral pathologists in resource limited settings. Due to lack of consensus on parameters for the diagnosis of AC, cases should thus be carefully analyzed and evaluated both clinically and histopathologically to detect subtle changes that may predict its aggressive behavior and diagnosis. This study is among the few in the scientific literature that documented higher frequency of secondary AC. In addition, little is known till date of its pathogenesis, although delay in seeking orthodox treatment by patients in low and middle income world setting may be an important contributory factor. Although scientific literatures has overwhelmingly favored the surgical management as the treatment of choice, novel emerging molecular therapies may soon overtake conventional therapies in an era of precision medicine.

What is known about this topic

  • Ameloblastic carcinoma is a rare malignant odontogenic neoplasm;
  • Ameloblastoma rarely exhibit malignant transformation to ameloblastic carcinoma;
  • Due to its rarity, ameloblastic carcinomas is often misdiagnosed as ameloblastoma.

What this study adds

  • This study reviews a large cohort of odontogenic tumors in sub-saharan Africa;
  • We have examine variations in presentation of ameloblastic carcinoma and provided tips on how to delineate it from other odontogenic tumors;
  • This study documents higher frequency of secondary ameloblastic carcinoma in our study population.

 

 

Competing interests Up    Down

The authors declare no competing interests.

 

 

Authors’ contributions Up    Down

Olujide Oladele Soyele conceptualized, designed, prepared and critically revised the manuscript, tables and figures. Kehinde Emmanuel Adebiyi, Olufunlola Motunrayo Adesina, Adeola Mofoluwake Ladeji, Adetayo Aborisade, Abiodun Olatunji, were involved in the design, data collection/analysis, and preparation of sections of the manuscripts. Henry Ademola Adeola was involved in the design, critical intellectual revision and finalization of the manuscript, figures and tables. All authors had final approval of the submitted and published versions.

 

 

Acknowledgments Up    Down

HAA thanks the South African Medical Research Council (SAMRC) for a mid-career scientist and Self-initiated research grant; and the South African National Research Foundation (NRF) for incentive and research development grants for rated researchers.

 

 

Tables and figures Up    Down

Table 1: clinicopathological review of 13 cases of ameloblastic carcinoma from OAUTHC, Ile-Ife between 2007 and 2016

Table 2: histopathological features of 13 patients with ameloblastic carcinoma

Figure 1: clinicopathological detail of 13 ameloblastic carcinoma cases. There was a male predominance among all ameloblastic carcinoma cases

Figure 2: A) the mandible was more affected than the maxilla and posterior region was common for both jaws; B) Also, swelling was a common feature of all cases with additional features such as pain and ulceration

Figure 3: clinical and radiological features of ameloblastic carcinoma. Ameloblastic carcinoma may presents as an aggressive expansile swelling of the jaws (A-C), and can present an exophytic extraoral mass (D). Radiographically, it can present as a jaw mass will an ill-defined border (E, F)

Figure 4: overview of histopathological finding in Ameloblastic carcinoma. Photomicrographs shows presence of cellular atypia with nuclear hyperchromatism, and cytoplasmic clearing (A). There were areas showing discohesion of the malignant cells, with invasion into the underlying deeper tissue (B). Perivascular and intravascular invasion by the discohesive Ameloblastic carcinomatous cells was observed (C). Also seen were islands of neoplastic malignant transformation of the peripheral basal cells with loss of polarity and presence of mitotic figures, as well as areas of the tumour islands undergoing necrosis (D). There was spindling of the cells with presence of cellular atypia involving the basal cells (E). Also seen were disruption of the basal cells with hyperchromatic nuclei, pleomorphic and clear cells; with presence of squamous cell carcinomatous transformation in the central area (F)

Figure 5: anaplastic transformation with mitotic cells and hyperchromatic nuclei were also observed

 

 

References Up    Down

  1. Mullapudi SV, Putcha UK, Boindala S. Odontogenic tumors and giant cell lesions of jaws-a nine year study. World journal of surgical oncology. 2011; 9: 68. PubMed | Google Scholar

  2. Avelar RL, Primo BT, Pinheiro-Nogueira CB, Studart-Soares EC, de Oliveira RB, Romulo de Medeiros J, Hernandez PA. Worldwide incidence of odontogenic tumors. The Journal of craniofacial surgery. 2011; 22(6): 2118-2123. PubMed | Google Scholar

  3. Lawal AO, Adisa AO, Olusanya AA. Odontogenic tumours: a review of 266 cases. Journal of clinical and experimental dentistry. 2013; 5(1): e13-17. PubMed | Google Scholar

  4. Buchner A, Merrell PW, Carpenter WM. Relative frequency of central odontogenic tumors: a study of 1,088 cases from Northern California and comparison to studies from other parts of the world. Journal of oral and maxillofacial surgery. 2006; 64(9): 1343-1352. PubMed | Google Scholar

  5. Ebenezer V, Ramalingam B. A cross-sectional survey of prevalence of odontogenic tumours. J Maxillofac Oral Surg. 2010; 9(4): 369-374. PubMed | Google Scholar

  6. McClary AC, West RB, McClary AC, Pollack JR, Fischbein NJ, Holsinger CF, Sunwoo J, Colevas AD, Sirjani D. Ameloblastoma: a clinical review and trends in management. European archives of oto-rhino-laryngology: official journal of the European Federation of Oto-Rhino-Laryngological Societies. 2016; 273(7): 1649-1661. PubMed | Google Scholar

  7. Jing W, Xuan M, Lin Y, Wu L, Liu L, Zheng X, Tang W, Qiao J, Tian W. Odontogenic tumours: a retrospective study of 1642 cases in a Chinese population. International journal of oral and maxillofacial surgery. 2007; 36(1): 20-25. PubMed | Google Scholar

  8. Karakida K, Aoki T, Sakamoto H, Takahashi M, Akamatsu T, Ogura G, Sekido Y, Ota Y. Ameloblastic carcinoma, secondary type: a case report. Oral surgery, oral medicine, oral pathology, oral radiology and endodontics. 2010; 110(6): e33-37. PubMed | Google Scholar

  9. Li J, Du H, Li P, Zhang J, Tian W, Tang W. Ameloblastic carcinoma: an analysis of 12 cases with a review of the literature. Oncology letters. 2014; 8(2): 914-920. PubMed | Google Scholar

  10. Panda S, Sahoo SR, Srivastav G, Padhiary S, Dhull KS, Aggarwal S. Pathogenesis and nomenclature of odontogenic carcinomas: revisited. Journal of oncology. 2014; 2014: 197425. PubMed | Google Scholar

  11. Yoon HJ, Hong SP, Lee JI, Lee SS, Hong SD. Ameloblastic carcinoma: an analysis of 6 cases with review of the literature. Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics. 2009; 108(6): 904-913. PubMed | Google Scholar

  12. Barnes L, Eveson JW, Reichart P, Sidransky D, Eds. World Health Organization Classification of Tumours. Pathology & Genetics of Head and Neck Tumours. In Lyon, France: IARCPress; 2005.

  13. Elzay RP. Primary intraosseous carcinoma of the jaws: review and update of odontogenic carcinomas. Oral Surg Oral Med Oral Pathol. 1982; 54(3): 299-303. PubMed | Google Scholar

  14. Pundir S, Saxena S, Rathod V, Aggrawal P. Ameloblastic carcinoma: Secondary dedifferentiated carcinoma of the mandible: report of a rare entity with a brief review. J Oral Maxillofac Pathol. 2011; 15(2): 201-204. PubMed | Google Scholar

  15. Athota A, Paladugu LV, Konidala U, Kkrishnan G. Ameloblastic carcinoma: a case series. Journal of Indian Academy of Oral Medicine and Radiology. 2015; 27(2): 302-306. Google Scholar

  16. Uzawa N, Suzuki M, Miura C, Tomomatsu N, Izumo T, Harada K. Primary ameloblastic carcinoma of the maxilla: a case report and literature review. Oncology letters. 2015; 9(1): 459-467. PubMed | Google Scholar

  17. Ladeinde AL, Ajayi OF, Ogunlewe MO, Adeyemo WL, Arotiba GT, Bamgbose BO, Akinwande JA. Odontogenic tumors: a review of 319 cases in a Nigerian teaching hospital. Oral surgery, oral medicine, oral pathology, oral radiology and endodontics. 2005; 99(2): 191-195. PubMed | Google Scholar

  18. Ponnam S, Srivastava G, Sudhakar GS. Ameloblastic Carcinoma with diverse histological features: a case report. International Journal of Oral & Maxillofacial Pathology. 2012; 3(1): 60-64. Google Scholar

  19. Martinez Martinez M, Mosqueda-Taylor A, Carlos R, Delgado-Azanero W, de Almeida OP. Malignant odontogenic tumors: a multicentric latin American study of 25 cases. Oral diseases. 2014; 20(4): 380-385. PubMed | Google Scholar

  20. Shafer W, Hine M, Levy B. A textbook of Oral Pathology, 4th edn. USA: WB Saunders; 1974.

  21. Gunaratne DA, Coleman HG, Lim L, Morgan GJ. Ameloblastic Carcinoma. Am J Case Rep. 2015; 16: 415-419. PubMed

  22. Zarbo R, Marunick MT, Johns R. Malignant ameloblastoma, spindle cell variant. Archives of pathology & laboratory medicine. 2003; 127(3): 352-355. PubMed | Google Scholar

  23. Avon SL, McComb J, Clokie C. Ameloblastic carcinoma: case report and literature review. J Can Dent Assoc. 2003; 69(9): 573-576. PubMed | Google Scholar

  24. Corio RL, Goldblatt LI, Edwards PA, Hartman KS. Ameloblastic carcinoma: a clinicopathologic study and assessment of eight cases. Oral Surg Oral Med Oral Pathol. 1987; 64(5): 570-576. PubMed | Google Scholar

  25. Nagai N, Takeshita N, Nagatsuka H, Inoue M, Nishijima K, Nojima T, Yamasaki M, Hoh C. Ameloblastic carcinoma: case report and review. Journal of oral pathology & medicine: official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology. 1991; 20(9): 460-463. PubMed | Google Scholar

  26. Benlyazid A, Lacroix-Triki M, Aziza R, Gomez-Brouchet A, Guichard M, Sarini J. Ameloblastic carcinoma of the maxilla: case report and review of the literature. Oral surgery, oral medicine, oral pathology, oral radiology and endodontics. 2007; 104(6): e17-24. PubMed | Google Scholar

  27. Akrish S, Buchner A, Shoshani Y, Vered M, Dayan D. Ameloblastic carcinoma: report of a new case, literature review, and comparison to ameloblastoma. Journal of oral and maxillofacial surgery. 2007; 65(4): 777-783. PubMed | Google Scholar

  28. Dhir K, Sciubba J, Tufano RP. Ameloblastic carcinoma of the maxilla. Oral Oncol. 2003; 39(7): 736-741. PubMed | Google Scholar

  29. Casaroto AR, Toledo GL, Filho JL, Soares CT, Capelari MM, Lara VS. Ameloblastic carcinoma, primary type: case report, immunohistochemical analysis and literature review. Anticancer research. 2012; 32(4): 1515-1525. PubMed | Google Scholar

  30. McNaught MJ, Turella SJ, Fallah DM, Demsar WJ. Spindle cell variant of ameloblastic carcinoma: a case report and review of literature. Military medicine. 2015; 180(5): e614-617. PubMed | Google Scholar

  31. Wu JY, Chi LH, Pemg BY, Lin YH. Ameloblastic carcinoma of the maxilla-case report. J Dent Sci. 2007; 2(3): 164-170.

  32. Mubeen K, Shakya HK, Jigna VR. Ameloblastic carcinoma of mandible: a rare case report with review of literature. Journal of clinical and experimental dentistry. 2010; 2: e83 e87. Google Scholar

  33. Ramesh M, Sekar B. Ameloblastic Carcinoma-Review and Histopathology of 5 Case. Oral & Maxillofacial Pathology Journal. 2011; 1(2): 154-160. Google Scholar

  34. Purmal K, Alam MK, Pohchi A, Rahman SA. Ameloblastic carcinoma of the mandible. Bangladesh J Med Sci. 2015; 14(2): 196-202. Google Scholar

  35. Kamath V, Satelur K, Yerlagudda K. Spindle cell variant of ameloblastic carcinoma arising from an unicystic amelobastoma: report of a rare case. Dental Research Journal. 2012; 9(3): 328-333. PubMed | Google Scholar

  36. Nodit L, Barnes L, Childers E, Finkelstein S, Swalsky P, Hunt J. Allelic loss of tumor suppressor genes in ameloblastic tumors. Mod Pathol. 2004; 17(9): 1062-1067. PubMed | Google Scholar

  37. Yoon HJ, Jo BC, Shin WJ, Cho YA, Lee JI, Hong SP, Hong SD. Comparative immunohistochemical study of ameloblastoma and ameloblastic carcinoma. Oral surgery, oral medicine, oral pathology, oral radiology and endodontics. 2011; 112(6): 767-776. PubMed | Google Scholar

  38. Abdelhamed F, El-Bolok AH, Hassan HI, Eldin EA. Expression of Midkine and Ki-67 Proteins in Ameloblastoma versus Ameloblastic Carcinoma According to their Clinicopathologic Parameters. Int J Adv Res. 2017; 5(4): 489-499.

  39. Lei Y, Jaradat JM, Owosho A, Adebiyi KE, Lybrand KS, Neville BW, Muller S, Bilodeau EA. Evaluation of SOX2 as a potential marker for ameloblastic carcinoma. Oral Surg Oral Med Oral Pathol Oral Radiol. 2014; 117(5): 608-616. e601. PubMed | Google Scholar

  40. Khojasteh A, Khodayari A, Rahimi F, Ghaderian MH, Jafarian M, Nayebi A, Akbarzadeh Najar R, Tabatabayipanah A, Jahangirnia A. Hypermethylation of p16 tumor-suppressor gene in ameloblastic carcinoma, ameloblastoma and dental follicles. Journal of oral and maxillofacial surgery. 2013; 71(1): 62-65. PubMed | Google Scholar

  41. Muller S, DeRose PB, Cohen C. DNA ploidy of ameloblastoma and ameloblastic carcinoma of the jaws: analysis by image and flow cytometry. Archives of pathology & laboratory medicine. 1993; 117(11): 1126-1131. PubMed | Google Scholar

  42. Makiguchi T, Yokoo S, Miyazaki H, Nobusawa A, Ogawa M, Hashikawa K, Terashi H. Treatment strategy of a huge ameloblastic carcinoma. The Journal of craniofacial surgery. 2013; 24(1): 287-290. PubMed | Google Scholar

  43. Ramadas K, Jose CC, Subhashini J, Chandi SM, Viswanathan FR. Pulmonary metastases from ameloblastoma of the mandible treated with cisplatin, adriamycin and cyclophosphamide. Cancer. 1990; 66(7): 1475-1479. PubMed | Google Scholar

  44. Jensen AD, Ecker S, Ellerbrock M, Nikoghosyan A, Debus J, Munter MW. Carbon ion therapy for ameloblastic carcinoma. Radiat Oncol. 2011; 6: 13. PubMed | Google Scholar

  45. Brown NA, Betz BL. Ameloblastoma: a review of recent molecular pathogenetic discoveries. Biomark Cancer. 2015; 7(Suppl 2): 19-24. PubMed | Google Scholar

  46. Kennedy WR, Werning JW, Kaye FJ, Mendenhall WM. Treatment of ameloblastoma and ameloblastic carcinoma with radiotherapy. European archives of oto-rhino-laryngology. 2016; 273(10): 3293-3297. PubMed | Google Scholar

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 


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Volume 34 (September - December 2019)

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Tables and figures

Table 1: clinicopathological review of 13 cases of ameloblastic carcinoma from OAUTHC, Ile-Ife between 2007 and 2016


Table 2: histopathological features of 13 patients with ameloblastic carcinoma


Figure 1: clinicopathological detail of 13 ameloblastic carcinoma cases. There was a male predominance among all ameloblastic carcinoma cases


Figure 2: A) the mandible was more affected than the maxilla and posterior region was common for both jaws; B) Also, swelling was a common feature of all cases with additional features such as pain and ulceration


Figure 3: clinical and radiological features of ameloblastic carcinoma. Ameloblastic carcinoma may presents as an aggressive expansile swelling of the jaws (A-C), and can present an exophytic extraoral mass (D). Radiographically, it can present as a jaw mass will an ill-defined border (E, F)


Figure 4: overview of histopathological finding in Ameloblastic carcinoma. Photomicrographs shows presence of cellular atypia with nuclear hyperchromatism, and cytoplasmic clearing (A). There were areas showing discohesion of the malignant cells, with invasion into the underlying deeper tissue (B). Perivascular and intravascular invasion by the discohesive Ameloblastic carcinomatous cells was observed (C). Also seen were islands of neoplastic malignant transformation of the peripheral basal cells with loss of polarity and presence of mitotic figures, as well as areas of the tumour islands undergoing necrosis (D). There was spindling of the cells with presence of cellular atypia involving the basal cells (E). Also seen were disruption of the basal cells with hyperchromatic nuclei, pleomorphic and clear cells; with presence of squamous cell carcinomatous transformation in the central area (F)


Figure 5: anaplastic transformation with mitotic cells and hyperchromatic nuclei were also observed


Keywords

Ameloblastoma
Ameloblastic carcinoma
Odontogenic tumors
Cytological atypia
Sub-saharan Africa

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